Characterization of molecular features underlying drought tolerance in Mozambique´s drought tolerant maize (Zea mays L.) varieties

Maize (Zea mays L.) is the most important staple food in Mozambique but its production is facing constraints such as drought, floods, cyclones, soil infertility, diseases, and pests, being drought one of the most limiting factor. To meet the challenge of producing under such conditions, it is important to invest on breeding programs to produce maize varieties that are resistant and adaptable to the present agroecological conditions. Maize is a C4 plant that evolved a biochemical mechanism of concentrating CO2 to overcome the oxygenase activity of Rubisco, which makes C4 plants photosynthetically more efficient than C3 plants. We Hypothesized that the levels of enzymes involved in photosynthesis (PEPC, PPDK, NADP-ME, and RubisCO) and their Post Translational Modifications (PTMs) correlate with the photosynthetic capacity of maize plants under drought stress. To examine their potential role as molecular markers for drought tolerance, we will make use of cutting-edge proteomics techniques. We will also provide data on agronomic performance and nutritional quality of the maize grain grown under the Greenhouse conditions in ITQB and under the local practices and agroecological conditions in Mozambique. We will use maize B73 as a control to test three varieties from Mozambique: Matuba, ZM523, and ZM 309. The main goal of the present project is to contribute with knowledge and important data that can be added to Mozambique’s maize breeding programs.